Characterization of Enterobacteria by Esterase Specific-Activity Profiles

Characterization of enterobacteria by esterase specific-activity profiles.

The spectrum of specific activities and the electrophoretic mobilities of esterases produced by 550 strains of Enterobacteriaceae belonging to 36 species and subclassified into six groups (group 1, Escherichia coli, Shigella and Escherichia hermanii; group 2, genus Salmonella and genus Citrobacter; group 3, genus Klebsiella and genus Enterobacter; group 4, genus Serratia and Serratia fonticola;...

The specific esterase activity of trypsin.

The discovery of relatively simple peptide derivatives as substrates for proteolytic enzymes has introduced a new approach to the problem of the specificity of this group of hydrolytic enzymes. Although crystalline trypsin is known to catalyze the hydrolysis of internal peptide bonds of proteins, its action toward internal peptide bondsof synthetic substrates has been demonstrated for only one ...

The specific esterase activity of carboxypeptidase.

It was recently found that the proteolytic enzymes trypsin (19 and chymotrypsin (2) possess specific esterase activity. In addition to catalyzing the hydrolysis of specific peptides (3-7), these enzymes also catalyze the hydrolysis of those esters which possess the structural environment of the specific peptides. In order to substantiate further the suggestion of Schwert et al. (1) that the spe...

Expanding the feruloyl esterase gene family of Aspergillus niger by characterization of a feruloyl esterase, FaeC.

A feruloyl esterase (FAE) from Aspergillus niger N402, FaeC was heterologously produced in Pichia pastoris X-33 in a yield of 10mg/L. FaeC was most active at pH 7.0 and 50°C, and showed broad substrate specificity and catalyzed the hydrolysis of methyl 3,4-dimethoxycinnamate, ethyl ferulate, methyl ferulate, methyl p-coumarate, ethyl coumarate, methyl sinapate, and methyl caffeate. The enzyme r...

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA